Figure 2.

The effect of hBD3 on the response to E. coli‐DNA in mouse FLDCs is TLR9 dependent. Mouse FLDCs from WT or TLR9 deficient (TLR9^−/−) mice were incubated with E. coli‐DNA with or without hBD3. Supernatants were collected and cytokine secretion was assayed by ELISA (A). Mouse FLDCs from WT or TLR9 deficient (TLR9^−/−) mice were incubated or transfected with E. coli‐ or self‐DNA. Supernatants were collected and cytokine secretion was assayed by ELISA (B). The response to TLR4 ligand LPS was intact in both WT and TLR9 –/–FLDC (and reduced in the presence of hBD3 as expected [31](Supporting Information Fig.6), Data are shown as mean ± SEM and are pooled from three or more independent experiments performed in triplicate. *p < 0.05, **p < 0.01 unpaired t‐test.