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Actin staining of MC3T3 cells cultured for 48 h on gelatin 10%, chitosan 0.10%/gelatin 10% and chitosan 0.50%/gelatin 10% (wt%) chemical and physical-co-chemical gels and TCP. Hydrogels were cross-linked with 20 U mTGase/ggelatin for 2 h at 37 °C (chemical) and 21 °C (physical-co-chemical). Nuclei: blue; F-actin: green. The scale bars represent 200 μm.
