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Effect of YM155 on survivin, p53, PUMA expression, and caspase-3 activity. SCC9 cells were treated with 0.01, 0.1, 1, and 10 ng/ml YM155 for 6, 12, and 24 h. (A) Survivin, p53, and PUMA protein expression was detected by Western blot assay; (B) Caspase-3 activity was detected by cleavage of the caspase-3 substrate vs. 0 ng/ml, * p<0.05, ** p<0.01 and *** p<0.001.
