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. 2017 Apr 24;23:1963–1972. doi: 10.12659/MSM.901643

Figure 3.

Figure 3

Effect of PUMA/caspase-3 on YM155-induced apoptosis and growth inhibition of SCC9 cells. SCC9 cells were transfected with PUMA siRNA or caspase-3 siRNA for 16 h, then we treated the transfected cells with YM155 (1 and 10 ng/ml) for 24 h. (A) PUMA protein expression was detected by Western blot assay; (B) Caspase-3 activity was detected by cleavage of the caspase-3 substrate. (C) Cell survival rate was detected by MTT assay; (D) Colony formation assay; (E) Apoptosis was determined by FACS analysis; (F) Apoptosis was determined by TUNEL assay vs. control, * p<0.05.