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. 2016 Nov 1;158(1):148–157. doi: 10.1210/en.2015-2048

Figure 2.

Figure 2.

Tyk2−/− mice expressing a Tyk2KD transgene in BAT and SKM display normal differentiation of classic brown fat cells (A) Schematic representation for generation of the transgenic mouse lines expressing Tyk2WT or Tyk2KD. (B) Representative western blot from 3-month-old mice showing expression of Tyk2 and kinase-inactive Tyk2 transgene in BAT. (C) Primary preadipocytes were isolated from transgenic lines and were subjected to in vitro differentiation and stained with Oil Red O. (D) Total RNA was isolated from in vitro differentiated primary brown preadipocytes and brown fat-specific RNA levels were measured using qPCR. (E) SKM-specific RNAs in differentiated primary brown adipocytes from transgenic lines (n = 4 independent experiments). P < 0.05 *compared toTyk2+/+TG, $compared with Tyk2−/−TG, #compared with Tyk2WT TG-Cre+, +compared with Tyk2KD TG-Cre+.