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. 2016 Nov 14;158(1):121–133. doi: 10.1210/en.2016-1818

Figure 6.

Figure 6.

Steroid levels and ovarian gene expression. (A) Plasma steroid levels during proestrus for mice with or without LH surges, before or after HFD. Data are shown as mean ± standard error of the mean (SEM). Estradiol and progesterone showed significant surge increases (P = 0.0027 and < 0.0001, respectively) by 2-way ANOVA, and the surge progesterone values showed a HFD-dependent decrease (P = 0.015). (B) Ovarian expression of steroid biosynthetic genes Cyp19a1, Hsd17b1, Star, Hsd3b1, Cyp11a1, and Cyp17a1 by qPCR. The Flox mice on LFD group is shown in white, the Flox mice on HFD group is shown in red, the BKO mice on LFD group is shown in green, and the BKO mice on HFD group is shown in blue. Cyp19a1 expression showed a significant interaction of diet and genotype (P = 0.0089) by 2-way ANOVA. Asterisks indicate statistical significance by testing after 2-way ANOVA as indicated (*P < 0.05).