Maintenance of follicle health in vitro in both the absence and presence of FSH. (A and A′) Representative images of Ki67 immunolabeling (green) of follicles following 96 hours in culture in the (A) absence (–FSH) and (A′) presence (A +10 ng/mL FSH). DAPI labeled nuclei, with no Ki67, are blue. (B and B′) Dual caspase (red) and TUNEL (green) labeling of follicles cultured for 96 hours in the (B) absence (–FSH) and (B′) presence (+10 ng/mL FSH). The percentage of positive nuclei in each treatment was quantified for (C) Ki67, (D) caspase, and (E) TUNEL, and compared between control and +FSH using an unpaired t test. *P < 0.05. Values are means and 95% confidence interval.