Fig 2.

Kinetic analysis of the expression of LAPTM5 mRNA (A) and LAPTM5 protein (B) during TPA-induced differentiation of U937 cells. U937 cells were treated with 32 nM TPA for the indicated time and then total RNA and cell lysates were extracted. For northern blot analysis, total RNA (10 μg) were electrophoresed, transferred, and probed with ³²P-labeled human LAPTM5, integrin α6 subunit, and 18S rRNA cDNA. After individual cell lysates were prepared, western blot analyses using anti-human LAPTM5, anti-COX-1, anti-COX-2, anti-APOC2, anti-NF-κB, anti-IκBα, anti-p47 phox, anti-cyclin A, and anti-GAPDH antibodies were performed as described in the Materials and Methods. A representative study is shown; two additional experiments yielded similar results.