Fig 3.

Northern blot analyses of LAPTM5-specific mRNA in various human normal tissues (A) and in various human malignant cell lines (B). Human multiple-tissue northern membranes and human immune system multiple-tissue northern membrane, each containing 2 μg of poly(A)⁺ RNA per lane, were sequentially hybridized using ³²P-labeled human LAPTM5 and β-actin cDNA. The northern blot contained 10 μg of total RNA in each lane, which was isolated from each cell line, was hybridized with ³²P-labeled human LAPTM5 and 18S rRNA cDNA.