Fig 7.

Flow cytometric analyses of Δψm loss (A), Bak activation (B), and western blot analyses of LAPTM5, Bcl-2, Bcl-xL, Mcl-1, Bak, Bax, Bid, Bim, and GAPDH (C), and activation of caspase-9, -8, and -3, PARP cleavage, and GAPDH (D) in HeLa cells ectopically expressing LAPTM5. HeLa cells untreated and transfected with pCAGGS or pCAGGS-LAPTM5 were harvested 48 h after transfection. Flow cytometric analyses of Bak activation and Δψm loss, and western blot analyses were performed as described in the Materials and Methods. Representative results are presented; two additional experiments yielded similar results.