Fig 9.
Effect of the pan-caspase inhibitor (z-VAD-fmk), the pan-cathepsin inhibitor (Cathepsin inhibitor I), and the cathepsin D inhibitor (Pepstatin A) on the ectopic LAPTM5 overexpression-induced apoptotic sub-G1 peak (A) and Δψm loss (B) in HeLa cells. After HeLa cells were transfected with pCAGGS or pCAGGS-LAPTM5 for 12 h, 30 μM z-VAD-fmk, 2.5 μM Cathepsin inhibitor I, or 1.0 μM Pepstatin A were added to the individual cells and incubated for an additional 36 h. The apoptotic sub-G1 peak and Δψm loss of the cells were analyzed using flow cytometry as described in the Materials and Methods. Representative results are presented; two additional experiments yielded similar results.