Fig 5. Loss of FAM83A expression induces apoptosis and inhibits PI3K/Akt signaling.

HCC1954 cells infected with shGFP, shA2, or shA6 were assessed for induction of apoptosis using etoposide treated HCC1954 shGFP cells as a positive control. (A) FAM83A knock-down resulted in reduced BrdU incorporation. (B) Cells were fixed and stained with propidium iodide for cell cycle analysis. FAM83A-knockdown cells have increased sub-G₁ peaks, indicative of cell death. (C) Live cells were stained with an antibody detecting the early apoptosis marker Annexin V. Knockdown of FAM83A resulted in elevated expression of Annexin V. (D) Immunoblot analysis of the apoptotic marker cleaved caspase 3 and cell signaling proteins shows FAM83A knockdown induces apoptosis while decreasing PI3K/Akt signaling.