Table 1. Primer sequences.
Target gene |
Sense primer | Antisense primer | Amplicon length |
---|---|---|---|
16S rRNA | AAGGCTATGACGGGTATCCG | GGGGGTTGCGCTCGTTGCGGG | 826 bp |
23S rRNA | CCACAGCGATGTGGTCTCAG | GGGACCGAACTGTCTCACGACG | 878 bp |
cagA | CCATCGATGGTAAAAATGTGAATCGT | CAGGTACCGCGGCCGCTTAAGATTTTTGGAAACCAC | 3700 bp |
cagA | ACCGCTCGAGAACCCTAGTCGGTAATGGG | ATATCGATTTAAGCCAATTTTTGATTCCTTG | 500 bp |
dupA | CTACAATATAGCTCTCAAAAG | AGCAATAAAACGCTTAAAAGTCTC | 2959 bp |
dupA | GCCAGAGATTTCAATGATGTC | AAAAATTTAGGCTCAAAGTCTG | 970 bp |
vacA s1 | CTGCTTGAATGCGCCAAAC | ATGGAAATACAACAAACACAC | 259 bp |
vacA s2 | CTGCTTGAATGCGCCAAAC | ATGGAAATACAACAAACACAC | 286 bp |
vacA m1 | GGTCAAAATGCGGTCATGG | CCATTGGTACCTGTAGAAAC | 290 bp |
vacA m2 | CATAACTAGCGCCTTGCAC | CATAACTAGCGCCTTGCAC | 352 bp |
atpA | GGACTAGCGTTAAACGCACG | CTTGAAACCGACAAGCCCAC | 841 bp |
efp | GGCAATTTGGATGAGCGAGCTC | CTTCACCTTTTCAAGATACTC | 559 bp |
mutY | GTGGTTGTAGYTGGAAACTTTACAC | CTTAAGCGTGTGTYTTTCTAGG | 676 bp |
ppa | GGAGATTGCAATGAATTTAGA | GTGGGGTTAARATCGTTAAATTG | 706 bp |
HptrpC | TAGAATGCAAAAAAGCATCGCCCTC | TAAGCCCGCACACTTTATTTTCGCC | 633 bp |
ureI | AGGTTATTCGTAAGGTGCG | GTTTAAATCCCTTAGATTGCC | 686 bp |
yphC | CACGCCTATTTTTTTGACTAAAAAC | CATTYACCCTCCCAATGATGC | 734 bp |