Skip to main content
. 2017 May 2;12(5):e0176793. doi: 10.1371/journal.pone.0176793

Fig 3. PA activation of NF-κB is TLR4 dependent.

Fig 3

(A-B) PA-induced phosphorylation of NF-κBp65 is TLR4 dependent. MoDCs were treated with 300μM PA for 20 min and analyzed for phospho NF-κBp65 (green) by fluorescence microscopy. (B) Quantitation of MFI phospho NF-κBp65 relative to DAPI (blue). (C) MoDC secretion of IL-1β is dependent on TLR4 signaling. MoDCs were treated with 150μM PA for 36hrs +/- CLI-095. The supernatant was analyzed for the concentration of IL1-β. (D) MoDC production of IL-1β mRNAis dependent on TLR4 signaling. MoDCs were treated with 150μM PA for 6hrs +/- Lipid IVa. mRNA was isolated and RT-qPCR was performed. (E) MoDC secretion of IL-1β is dependent on TLR4 signaling. MoDCs were treated with 150μM PA for 36hrs +/- Lipid IVa. The supernatant was analyzed for the concentration of IL1-β. N = 3.