Fig 3. Ubiquitously reduced Cln2 transcript abundance in Cln2^R207X/R207X mice.

Quantitative real-time PCR was used to measure endogenous levels of the Cln2 transcript from five different tissues obtained from 1-month-old Cln2^+/+ (n = 3) and Cln2^R207X/R207X (n = 3) mice. Three technical replicates were performed using the three biological samples obtained from Cln2^+/+ and Cln2^R207X/R207X mice. Cln2 transcript levels were normalized to Gapdh expression. Columns and bars represent mean ± SEM. Statistical significance was determined using an unpaired t-test (**p < 0.01 and **** p < 0.0001).