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. 2017 Apr 15;220(8):1405–1410. doi: 10.1242/jeb.149369

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© 2017. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 1. — Original VAChT allele and FRT-STOP-FRT-VAChT::HA allele. FRT-flanked transcriptional stop signal in the 5′ UTR constrains the expression of VAChT::HA. Removal of the stop cassette requires FLP recombinase, introduced by the Gal4/UAS system. Expression of the VAChT::HA is therefore restricted to cells with active endogenous regulatory sequences of VAChT that are, in addition, part of the Gal4 expression pattern. The 3xP3-dsRed sequence encodes a screenable eye marker. ORF, open reading frame.