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. 2017 Apr 15;220(8):1405–1410. doi: 10.1242/jeb.149369

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© 2017. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 3. — The Mi1 and Tm3 neurons are cholinergic. The VAChT::HA can be found in all layers of the medulla and lobula in which Mi1 (A) and Tm3 (B) neurons laterally extend their processes (white arrowheads). The subcellular localization of the presynaptic marker nsyb::GFP in the Mi1 (C) and Tm3 (D) neurons (white arrowheads) corresponds to that of VAChT::HA. The insets in C and D show close-ups of the medulla regions containing presynaptic release sites of Mi1 and Tm3 neurons labeled by VAChT::HA and nsyb::GFP. Anti-ChAT staining co-localizes with the GFP-labeled somatic cytoplasmic membrane of the Mi1 (E) and Tm3 (F) neurons. White asterisks mark the position of the GFP-labeled cell bodies of the Mi1 and Tm3 neurons. All scale bars: 20 µm. Me, medulla; Lo, lobula; LP, lobula plate.