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. 2017 Apr 15;220(8):1481–1489. doi: 10.1242/jeb.151506

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© 2017. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 1. — Regulation of miR-29a and stearoyl-CoA desaturase (SCD) by normal fat diet (NFD) and high fat diet (HFD) groups and expression pattern of miR-29a in genetically improved farmed tilapia (GIFT) extracted from different tissues (n=9 replicates per group). (A) The expression of miR-29a was quantified by qRT-PCR from NFD and HFD in GIFT liver. The values are expressed as the relative ratio with U6 as reference gene. **P<0.01. (B) The expression of SCD was quantified by qRT-PCR from NFD- and HFD-fed GIFT tilapia liver. Values are expressed as the relative ratio with 18S rRNA as the reference gene. (C) Expression pattern of miR-29a in GIFT. miRNA samples were extracted from different tissues, including muscle, liver, kidney, spleen, blood and gut. miRNA expression was detected by qRT-PCR.