Table 1. Primer list.
| Strain | Primer | Sequence |
| MH15 |
mh15-5' | CGGAATATGGGACTACTTCGCGCAACAGTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAGTGGTTGACGAGACTACTTCGCGCAAGGAAGCTTCGTACGCTGCAGG |
| mh15-3' | ACACTCTATAAGGCCAAATGTACAAACACATCTTCCCAATATCCGTCACCACGTACTTCAGCATAATTATTAGGCCACTAGTGGATCTGA | |
| M18 | m18-5' | AATATGGGACTACTTCGCGCAACAGTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAGTGGTTGACGATGGGACTACTTCGCGCAAGGAAGCTTCGTACGCTGCAGG |
| m18-3' | ACACTCTATAAGGCCAAATGTACAAACACATCTTCCCAATATCCGTCACCACGTACTTCAGCATAATTATTAGGCCACTAGTGGATCTGA | |
| SS2 | ssa1 | GACGCTCTTCACCGCCCACTTCTAAGCTGATTTCAATCTC |
| ssa2 | GACGCTCTTCTACTCGTCAACCACTCTACAAAACC | |
| ssa3 | GACGCTCTTCTAGTCTGGAAGTCAAAATACTCAGTTTC | |
| ssa4 | GACGCTCTTCCATGCCGTCGACCTGCAGCGTACGAAGCTTCCGCGCGAAGTAGTCCCATATTCCGTG | |
| SS527 | 527-F | TCAGTATAGCTATCCTATTTGA |
| 527-R | GGTAAACTTGATATTGTTATAAAG | |
| Pho87-HYG-F | AATTGGCTTAGGCAATGGATACTTGAAATATTCAATTTGAAGCTTTTCTTGCATAAGTTTCTCAGCTTCATCTTCAATAGGAAGCTTCGTACGCTGCAGG | |
| TAF2-3-R | GTCGTACCTGCACGACCACATTGTTTGGGAAAGAAGCAACACATGGAAAGACATGTTGGAGCGCCTCTCTTCGCAAAATAACGGGTAAACTTGATATTGTTATAA | |
|
URA3 Reporter Position (kb)a |
Primer | Sequence |
| C-20 | SS1-F | TATGACAAGTTGCTTCCTGTCTTTATGGTCTGAGAGCAGTAAAAAAGCTTCATGGTAACTTTATTAACATACCCTACAACCTTAGTAGTTGGT |
| SS1-R | ATGCATGGCTTAAAGAAAATACATTGCTGAAGCAAAAGCTCACCAAAAATTTGCGTTGCAAAGCTTTGTTTGCAGTTTTGCTGGCCGCATCT | |
| C-7.2 | SS2-F | TCTCTAAACTATGATTTGGACACATTTACGGATAGATTACCTAACGCTGGAGAAACTTTAGTTTTGCTGGCCGCATCT |
|
URA3 Reporter Position (kb)a |
Primer | Sequence |
| C-7.2 | SS2-R | ATGGCCGCAGCTTGGTTCAATCCCTTACCACCAGCATGTGTTTCGAAGTGGTTAGCCCTGCTACAACCTTAGTAGTTG. |
| C-5.8 | SS3-F | TTGTCACAGGTGTTTTGTTGGGTGTTAAAACTTTCAATGACCCTGTCGAACACCGGTGTATAGTTTTGCTGGCCGCATCT |
| SS3-R | TGTGATGTGTAATGGAATGGCTTCACTAGCCCATAAGAAAGCACAGCATTCTACCAATGCCCTACAACCTTAGTAGTTGG | |
| T-7.1 | SS4-F | TGCATTGTTATTCCTTCGAAACCAGCAGTAATAAGTCGTCCTGAGAACGATGTAGATTTACTACAACCTTAGTAGTTGGT |
| SS4-R | CAAGAAGAAGGACGACTTTAAGATGGAAGGAGGTGATTTAGAGTACCAAC ATGTAAAGATCAGTTTTGCTGGCCGCATCT | |
| T-9.1 | SS5-F | AAAAACTAATGTTGACATGACATTATGCAATAATTTATTTATGAGAGAAAGAGAAAAACACTACAACCTTAGTAGTTGGT |
| SS5-R | TGTCACACTACCTCTTGGTGAGATTCGATTGAAGAGGCGTGATTTTATGAAAAATTTGTAGAGTTTTGCTGGCCGCATCT | |
| T-11.5 | SS6-F | TATACACGCACTATTTTTCTTATATACAGGAGATGGGTGGCCACAGAACCCGCGCCTAGCCTACAACCTTAGTAGTTGGT |
| SS6-R | TACATCAGAGGTACAAGGTTGGATGTCGGCGACCTCGAGGCAGGTCTAAGAAGAAAGAAATAGAGTTTTGCTGGCCGCATCT | |
| T-14.5 | SS7-F | CTTTTCGTTTGGTGTCTGTTCAGGGCCCAGTATCATTATGACGTTGTACCTGACCTTATGACTACAACCTTAGTAGTTGGT |
| SS7-R | CCATTCCCTATTGTATATCTATCAAGGGCTTGCGAGGGACACACGTGGTATGGTGGCAGTAGTTTTGCTGGCCGCATCT | |
| Sequencing Location (kb)a |
Primers |
Sequence |
| T-7.1 | SEQ-1 | CAAGAAGAAGGACGACTTTAAGATG |
| SEQ-2 | TGCATTGTTATTCCTTCGAAACCAGC | |
| SEQ-3 | AGTCAAATTGCAGTACTCT | |
| SEQ-4 | ATTCGTAATGTCTGCCCAT | |
| C-5.8 | SEQ-3 | AGTCAAATTGCAGTACTCT |
| Sequencing Location (kb)a |
Primers |
Sequence |
| C-5.8 | SEQ-4 | ATTCGTAATGTCTGCCCAT |
| SEQ-5 | GGACAGTTAGAAGGGGAACCAATAAAC | |
| SEQ-6 | AATTGAAAATTTGGCAGTTCCTAAGCTA |
a Depicts the position of the URA3 reporter gene from the break site in kilobases
“T” represents the telomeric side of the HO-break site. “C” refers to the centromeric side of the HO-break site.