Regulation of arcuate neuropeptide and receptor genes by EB and HFD. (A) Pomc, (B) Npy, (C) Agrp, (D) Ghsr, (E) Lepr, (F) Insr, (G) 5ht2cr, and (H) Pparγ expression calculated within each genotype. Data were analyzed by a 2-way ANOVA with post hoc Newman-Keuls test within each genotype. Sample size was 8 per group. Capped lines denote comparisons within genotype between steroid (oil vs EB) and diet (LFD vs HFD) combinations (a, P < 0.05; b, P < 0.01; c, P < 0.001; d, P < 0.0001).