Figure 5.

Combinatorial vemurafenib (PLX) and hydroxychloroquine (HCQ) treatment reduced tumor growth in xenograft models. (A) Tumor growth curves of BCPAP xenografts following initiation of treatment with vehicle (n = 8), HCQ (150 mg/kg per day, n = 8), PLX4720 (30 mg/kg per day, n = 8), or a combination of PLX4720 and HCQ (n = 8) by oral gavage once daily (6 days/week) for 2 weeks. Tumor volume was evaluated every other day. The relative tumor volumes were normalized to their original sizes and plotted as the fold change relative to day 0. (B) Statistical analysis of tumor volume among different groups at indicated time course of treatment. (C) Representative transition electron micrographs of BCPAP xenografts under the treatment with vehicle (DMSO) or vemurafenib (PLX). Black arrows denote AVs. Magnification ×5000 to 50,000. Error bars, standard error of the mean of tumor volumes in each group.