Figure 1.

Expression of the transgene in CaMKII-tTA⁺/TBRIIΔk⁺ mice. CaMKII-tTA⁺/TBRIIΔk⁺ mice were generated by crossing CaMkII-tTA⁺ mice with tet-O-TBRIIΔk⁺ mice expressing a kinase-deficient TGF-β type II receptor. CaMKII-tTA⁺/TβRIIΔk⁺ and CaMKII-tTA⁺/TBRIIΔk⁻ (as control) mice were killed at 2 months of age and their brains were sectioned sagittally. The transgene expression was visualized by immunostaining with an antibody against a flag epitope (present in TBRIIΔk). A, A representative image of flag immunoreactivity in different brain regions. Note the strong flag immunoreactivity in the striatum. B, Higher-magnification images showing flag immunoreactivity in the hippocampus, cortex, and striatum in the CaMKII-tTA⁺/TBRIIΔk⁺ mice (left panels), compared with CaMKII-tTA⁺/TBRIIΔk⁻ mice (right panels). Scale bar, 50 μm. C, Tissue lysates from different brain regions were subjected to Western blot analysis using anti-flag and β-actin antibodies.