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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: Int J Biol Macromol. 2016 Dec 29;97:141–147. doi: 10.1016/j.ijbiomac.2016.12.072

Figure 7.

Figure 7

Monocyte adherence and migration into collagen matrix in response to MCP-1 gradients. Collagen matrices were pretreated with 25 ng/ml MCP-1 and incubated for 24 h. Samples with no wash (total MCP-1), washed 6X (bound MCP-1), or washed 6X and treated with an excess of MCP-1 neutralizing antibody (neutralizing Ab) were compared. A sample without the addition of MCP-1 was used as a negative control. Human monocytes (1.5*105 cells/cm2 in 0.5 ml media) were added on top of the collagen matrices and the wells were incubated at standard conditions for 2 h. At the end of the incubation time, the top surface was rinsed and the number of monocytes that adhered or migrated in the collagen matrix was counted. Values are presented as mean ± SD of the percent of attached and migrated monocytes normalized to the initial number of monocytes added to each well; n=3; *p < 0.05 for change in percentage of adhered and migrated monocytes compared to control samples lacking MCP-1 treatment.