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. 2017 May 1;214(5):1351–1370. doi: 10.1084/jem.20150237

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© 2017 Freeman et al.

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Figure 3. — NLRC4 is expressed in mouse CNS during neuroinflammation and in human brain tissue from MS patients. (A) WT mice brains were examined for NLRC4 expression by IHC using a mouse NLRC4 antibody. NLRC4 expression (red) was detected after 4 wk of cuprizone-induced demyelination in WT (Nlrc4^+/+) but not in Nlrc4^−/− mice. (B) The corpus callosum from 4-wk cuprizone–treated WT mice was stained with anti-NLRC4 (top, green; bottom, red), and RCA (red) was used to detect microglia (top), and GFAP (green) was used to detect astrocytes (bottom) at the corpus callosum. DAPI (blue) was used to label nuclei. Overlay (yellow) indicates colocalization of NLRC4 with the cell-specific biomarkers. (C and D) Chronic MS brain tissues were obtained from the UCLA HBSFRC. The corpus callosum sections were stained for NLRC4 using a human NLRC4 antibody (αNLRC4), as this is the region studied in the cuprizone model. Astrocytes (C) and microglia (D) were detected using GFAP and RCA antibodies. The overlay was performed to detect colocalization of proteins. All data are representative of at least two independent experiments. Bars, 50 µM.