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. 2017 May 1;214(5):1313–1331. doi: 10.1084/jem.20161076

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© 2017 Kitada et al.

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Figure 9. — BATF2 down-regulates Il23a expression through its interaction with c-JUN. (A) Enrichment analysis of the upstream regulators of genes up-regulated in Batf2^−/− BMMφs. (B) Luciferase activity of Il23a promoter. Data are representative of three independent experiments. Graph show mean values ± SD of triplicate wells. *, P < 0.001. RLU, relative light unit. (C) Coimmunoprecipitation (IP) assay with anti–c-JUN antibody for immunoprecipitation and the indicated antibodies for immunoblotting. WCL, whole cell lysate. (D) Luciferase activity of Il23a promoter in response to LPS. Data are representative of three independent experiments. Mean values ± SD for triplicate wells are shown. *, P < 0.01; n.s., not significant. (E) Coimmunoprecipitation assay with anti–c-JUN antibody for immunoprecipitation and the indicated antibodies for immunoblotting. (F) Batf2^−/− BMMφs were transfected with the indicated vectors by nucleofection and, after 18 h, stimulated with LPS for 1 h, and their expression of Il23a was analyzed. Data are representative of three independent experiments. Graphs show mean values ± SD of triplicate measurements. *, P < 0.01. (G) ChIP assay for the Il23a promoter of AP-1–binding site was performed with anti–ATF-2 antibody using wild-type and Batf2^−/− MEFs. Means ± SD of triplicate PCRs on identical samples. *, P < 0.03; **, P < 0.005; n.s., not significant. These values are representative of three independent experiments.