Skip to main content
. 2017 May 1;214(5):1297–1311. doi: 10.1084/jem.20161141

Figure 4.

Figure 4.

BA spore–induced macrophage gene expression depends on endosomal nucleic acid–sensing TLRs. (A–C) Macrophages from the indicated mice (A and C) or WT mouse macrophages (B) were exposed to BA spores at moi of 3. Relative RNA amounts for spore-induced genes at the indicated time points after exposure were determined by qPCR, and plotted on a linear scale. Macrophages were pretreated with the following pharmacological agents 1 h before spore exposure (B): bafilomycin A1 (Bm), dynasore (Ds), z-FA-cmk (FA), and CA074-Me (CA). (D) Macrophages derived from control and UNC93B1-KO THP-1 cells were exposed to BA spores at moi of 3. Relative RNA amounts for spore-induced genes at the indicated time points after exposure were determined by qPCR, and then plotted on a linear scale. Data are representative of three experiments with similar results (A) or from one experiment (B–D).