Figure 1.

Antibody-mediated neutrophil depletion results in increased mortality after LPS injection. (A–C) Representative flow cytometry profile (A) and quantification of CD62L (B) and CD11b (C) by geometric mean fluorescence intensity (GeoMean) on Ly6G⁺ CD11b⁺ blood neutrophils 6 h after LPS injection at the indicated concentrations. Red areas outlined in A provide a visual indication of CD62L and CD11b on the neutrophil population from the control 0 group. B and C show values from individual mice; bars indicate means ± SEM pooled from two independent experiments. **, P < 0.01; ***, P < 0.001 versus control 0 group by two-tailed Mann–Whitney U test. (D–I) Changes in body temperature (Δ°C [mean ± SEM]) and survival (percentage of live animals) after LPS injection in C57BL/6J mice treated i.p. with anti-Ly6G (D and E), anti–Gr-1 (F–I) neutrophil-depleting antibodies, or respective isotype control antibodies. Data in D–G are pooled from three independent experiments performed at Stanford University (total n = 10–12/group). Data in H are pooled from three independent experiments performed in the Institut Pasteur conventional SPF animal facility (total n = 21–26/group). Data in I are pooled from two independent experiments performed in the Institut Pasteur GF animal facility (total n = 18/group). **, P < 0.01; ***, P < 0.001 versus the corresponding isotype control group by Mantel–Cox log-rank test. Arrows in D–I indicate days of i.p. injection of the neutrophil-depleting or isotype control antibodies.