Appendix 4 – Table 1.
Object- versus pixel-based colocalization.
Object-based methods are best applicable for the colocalization of binary signals, but not/less for applications, in which colocalization accounts for coupling of molecular counts on a continuous spectrum.
| Pixel based | Object based |
|---|---|
| Pixel-wise correlation | Spatial colocalization |
| Limited to object-detectable data | |
| Suffer from background signal | |
| Highly altered by detection accuracy | |
| Affected by confounding factors (e.g., CCP size) | Lose the information at the detected objects |