Table 2.
Mediator release from PEMs exposed to purified histones (37° C, 4 h incubation)
| Cytokine/chemokine | H1 | H2A | H2B | H3 | H4 |
|---|---|---|---|---|---|
| IL-6 | |||||
| EC50, µg/mL | >66 | 14 | 10 | >67 | >59 |
| Peak levels, pg/mL | <2 | 609 | 414 | <5 | <7 |
| TNF | |||||
| EC50, µg/mL | 6 | 5 | 5 | >28 | >64 |
| Peak levels, pg/mL | 81 | 1,554 | 1,636 | 121 | 616 |
| IL-1β | |||||
| EC50, µg/mL | >42 | >38 | >47 | >46 | >100 |
| Peak levels, pg/mL | 91 | 183 | 248 | 103 | 0 |
| CXCL1 | |||||
| EC50, µg/mL | 10.2 | 24 | 10 | >50 | >50 |
| Peak levels, pg/mL | 241 | 18,237 | 12,125 | 1,940 | 1,504 |
| CXCL2 | |||||
| EC50, µg/mL | 10 | 25 | 5 | >50 | >50 |
| Peak levels, pg/mL | 1,188 | 17,796 | 19,752 | 4,650 | 3,515 |
| CCL3 | |||||
| EC50, µg/mL | 26 | 5 | 5 | >50 | >50 |
| Peak levels, pg/mL | 47 | 21,684 | 82,712 | 321 | 473 |
EC50, effective concentration causing 50% of the maximal response.