Figure 2. LC-MS/MS was used to measure NNMT activity.
The accurate mass LC-MS Q-TOF and LC-MS QQQ instruments were used in combination with the Mass Hunter qualitative analysis (B.03.01) software to identify the concentrations of NA and MNA. The smaller boxes adjacent to the ion chromatogram represent the target analysis MS/MS product ion spectrums of NA and MNA. The table within the ion chromatogram represents the intracellular concentration of NA and MNA in the patient-derived primary, commercially available, and NNMT isogenic cell lines.
