Figure 4. NNMT silencing attenuates the activation of pro-survival kinases and induces apoptosis following radiation treatment.
(A–D) Western Blot analysis of (A) the activation of the Akt and MAPK kinases in the WT, KD, and OE cell lines following 6 Gy radiation over a 5 day time period, (B) the basal NNMT expression levels in the isogenic U87 cell lines, (C) the activation of the Akt and MAPK kinases in the NT and KD cell lines following 6 Gy radiation over a 6 hour time period, (D) Caspase-3, PARP, and the apoptotic marker cleaved PARP in the NT, KD, and OE cell lines following 6 Gy radiation over a 24 hour period.
