Figure 6. NNMT silencing decreases the methylation potential and schematic illustration.
(A) Western blot analysis was used to determine the change in PP2A methylation/activation following different concentrations of PPZ and OA in the WT, KD, and OE cell lines.
(B) Western analysis of NNMT expression and Akt and p44/42 MAPK activation following the addition of OA with and without the addition of PPZ.
(C) A schematic illustrating the in cancer cells, the upregulation of NNMT utilizes an increased amount of SAM, directly modulating the expression of pro-survival kinases by influencing the activation of phosphatases.
