Fig. 2.

SMVT transcript and protein studies. (a) SMVT mRNA expression: real-time PCR was performed using total RNA isolated from lymphocytes. The level of hSMVT expression was normalized relative to β-actin as described in “Materials and Methods”. Data are mean ± SE of three independent experiments. (b) SMVT protein expression: total protein isolated from the lymphocytes was used for western blotting. The blot was probed with anti-SMVT antibody and expression was normalized relative to β-actin