Figure 3.

miR-142-5p negatively regulated axon elongation in cortical neurons. (A) Two-color immunofluorescence staining of anti-MAP2 (red) and Hoechst 33342 (blue) on a portion of the cerebral cortex in frozen mouse brain sections. Most red staining of the axonal flow of PHEV-infected mice shows spreading of the disorder (inside the box). (B) PHEV-infected neurons grew significantly shortened axons (upper panel), while normal control neurons showed bipolar morphology, with two main long axons. Neurons transfected with miR-142-5p inhibitors had similar morphologies as normal neurons. All mimic-transfected neurons had shorter axons. Antibody straining of MAP2 (green) identified dendrites. (C) Histograms represent the percentages of neurons with different lengths after PHEV infection, mimics/inhibitors transfection, and control treatment. The y-axis represents the percentage of neurons, and the x-axis represents the length of axons grouped into five columns.