Table 1B.

Alternative of viral vector-mediates gene transfection.

Non-virus	Capability	Delivery method	Benefit compare to viral vector-mediates gene transfection
Electroporation	>10 kb	–	Do not integrate/time saving/cost low
			Less toxicity/less immunity
			Higher efficiency/bigger capacity
CATIONIC LIPID MEDIATES DELIVERY
DNA	Unlimited		Longer duration
		Stereotaxic injection	Higher mean level of expression per cell
RNA			Unnecessary of transit across the nuclei membrane
			Onset of expression is faster than DNA
			Preferable in some clinical applications
POLYETHYLENIMINE
	Unlimited	Bone-marrow derived macrophage system	Less cytotoxicity
			Increased cellular uptake and stability
			Non-invasive imaging for tracking
		Ventricular delivery
DENDRIGRAFTED POLY-L-LYSINE
	Unlimited	Ventricular delivery	Cross the BBB by specific receptor mediated transcytosis
			Targeted nanoparticles could accumulate in brain more efficiently
CHITOSAN WITH GRAFT WITH PEI
	Unlimited	Stereotaxic injection	Improved water solubility
			Low toxicity
			High transfection efficiency