Figure 2.

L. monocytogenes MVs production in vivo. (A) Thin section electron micrograph of murine RAW264.7 macrophages infected with EGDeΔhly L. monocytogenes grown to exponential phase for 90 min at the MOI 20:1. Micrograph shows phagosome containing intracellular bacterium releasing MVs (shown with arrows). Scale bar: 500 nm. (B) Immunogold thin section electron micrograph of murine RAW264.7 macrophages infected with the EGDeΔhly L. monocytogenes. MVs released from bacterial surface inside the phagosome were detected using anti-Listeria O antiserum type I followed by protein A conjugated 10 nm gold nanoparticles (indicated with arrows). Scale bar: 500 nm. (C) Electron micrograph showing a magnified area in the square of Panel (B).