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. 2017 May 3;8:708. doi: 10.3389/fmicb.2017.00708

Table 5.

Expression of distinct OmpA variants in the same genetic background significantly impacts attachment to corn stover.

Template Strain (polymorphism pattern) MG1655 (I, α) 57 (II, α) 53 (III, γ) 111 (IV, β) 62 (V, α) 44 (VI, α) 127 (VII, β)
% Attached, average ± SE (n = 3) 0 ± 0 −8 ±11 −24 ± 2 −35 ± 7 14 ± 4 11 ± 1 18 ± 4
P-VALUES, STUDENT'S T-TEST
MG1655 (I, α) 0.00049 0.0058 0.020 0.0011 0.013
57 (II, α)
53 (III, γ) 0.00049 0.00098 0.00019 0.00099
111 (IV, β) 0.0058 0.0028 0.0022 0.0024
62 (V, α) 0.020 0.00098 0.0028
44 (VI, α) 0.0011 0.00019 0.0022
127 (VII, β) 0.013 0.00099 0.0024
OmpA POLYMORPHISMS
Loop 1 N P P N D D D
Loop 2 SVE DNI DNI SVE SVE DNI SVE
93 I I I V V V V
Loop 3 SNVYGKN ANVPGGASFKD ANVPGGASFKD SNFDGKN AHNNVTGESEKN SNVYGKN SNFDGKN
129 Y Y Y Y W Y Y
Loop 4 H N H H H H H
161 M L M M L L M
175 A A V A A A A
203 N N T T N N T
251 G G A G G G G

OmpA was cloned from the indicated template strain and expressed from the pGEN-MCS expression vector in MG1655 ΔompA. The one-way ANOVA P-value was 3.9 × 10−5. Amino acids are color-coded according to the convention described in the legend of Figure 4. P values are shown only when the value was less than 0.05.

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