Table 5.
Expression of distinct OmpA variants in the same genetic background significantly impacts attachment to corn stover.
Template Strain (polymorphism pattern) | MG1655 (I, α) | 57 (II, α) | 53 (III, γ) | 111 (IV, β) | 62 (V, α) | 44 (VI, α) | 127 (VII, β) |
---|---|---|---|---|---|---|---|
% Attached, average ± SE (n = 3) | 0 ± 0 | −8 ±11 | −24 ± 2 | −35 ± 7 | 14 ± 4 | 11 ± 1 | 18 ± 4 |
P-VALUES, STUDENT'S T-TEST | |||||||
MG1655 (I, α) | 0.00049 | 0.0058 | 0.020 | 0.0011 | 0.013 | ||
57 (II, α) | |||||||
53 (III, γ) | 0.00049 | 0.00098 | 0.00019 | 0.00099 | |||
111 (IV, β) | 0.0058 | 0.0028 | 0.0022 | 0.0024 | |||
62 (V, α) | 0.020 | 0.00098 | 0.0028 | ||||
44 (VI, α) | 0.0011 | 0.00019 | 0.0022 | ||||
127 (VII, β) | 0.013 | 0.00099 | 0.0024 | ||||
OmpA POLYMORPHISMS | |||||||
Loop 1 | N | P | P | N | D | D | D |
Loop 2 | V | I | I | V | V | I | V |
93 | I | I | I | V | V | V | V |
Loop 3 | V | AVPAF | AVPAF | F | AV | V | F |
129 | Y | Y | Y | Y | W | Y | Y |
Loop 4 | H | N | H | H | H | H | H |
161 | M | L | M | M | L | L | M |
175 | A | A | V | A | A | A | A |
203 | N | N | T | T | N | N | T |
251 | G | G | A | G | G | G | G |
OmpA was cloned from the indicated template strain and expressed from the pGEN-MCS expression vector in MG1655 ΔompA. The one-way ANOVA P-value was 3.9 × 10−5. Amino acids are color-coded according to the convention described in the legend of Figure 4. P values are shown only when the value was less than 0.05.