Figure 1.

Fusion Maximum Intensity Projection (MIP) of ⁸²Rb-PET/CT scan of a Male Sprague-Dawley rat after myocardial infarction LAD ligation, showing excellent cardiac uptake with practically no background uptake apart from clearance through the kidneys. The animal which was anesthetized using 4 % Sevoflurane in 35 % O_2, had a catheter placed in the tail vein, and was placed in a Siemens Inveon pre-clinical PET/CT scanner (Siemens, Knoxville, US). PET acquisition was performed for 5 minutes, with infusion of approximately 40 MBq ⁸²Rb in 2 ml saline over 4-5 seconds from a clinically approved ⁸²Rb generator (CardioGen-82, Bracco Diagnostics Inc., US) simultaneous with PET imaging, followed by a CT scan for anatomical co-registration and attenuation correction. PET list-mode data were histogrammed into two timeframes, the first 45 seconds and the remaining 255 seconds (the latter is shown here), and images were reconstructed using scatter and attenuation corrected OP-MAP / 3D-OSEM with 3 mm requested resolution, 18 and 2 subsets, respectively. All experiments were approved by the Danish Animal Experiments Inspectorate (Permit No. 2012-15-2934-00064)