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. 2017 Apr 26;12:3375–3393. doi: 10.2147/IJN.S126310

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© 2017 Zhu et al. This work is published and licensed by Dove Medical Press Limited

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Cellular uptake and distribution of the siRNA–PTX/LDL–NSC–SS–UA micelles in MCF-7/Taxol cells in vitro.

Notes: (A) Intracellular uptake and location of siRNA–PTX/LDL–NSC–SS–UA micelles in MCF-7/Taxol cells. (B) Dynamic uptake images of micelles and kinetics of fluorescence intensity. (C) Quantitative uptake of micelles in MCF-7/Taxol cells with two-color flow cytometry. (D) Micelle uptake kinetic parameters obtained by the change in fluorescence over time. Magnification 40×.

Abbreviations: Cy3, cyanine 3; C6, coumarin 6; siRNA–PTX/LDL–NSC–SS–UA, small interfering RNA–paclitaxel/low-density lipoprotein–N-succinyl chitosan–cystamine–urocanic acid; DIC, differential interference contrast; FITC, fluorescein isothiocyanate; DAPI, 4′,6-diamidino-2-phenylindole.