Figure 2.

Development of FR-targeted MNT (MNT-PEG-FA).

Notes: (A) Schematic presentation of MNT derivatization with FA moiety with a SDS-PAGE gel under nonreducing conditions of MNT and the resulting FR-targeted MNT-PEG-FA. (B) Schematic presentation of MNT-PEG-FA labeling with Auger electron emitter ¹¹¹In with autoradiography of SDS-PAGE gels of ¹¹¹In-MNT-PEG-FA. Labels on the left indicate the bands from ColorBurst™ Electrophoresis Markers.

Abbreviations: DTox, diphtheria toxin translocation domain; FA, folic acid; HMP, hemoglobin-like protein of E. coli; MNTs, modular nanotransporters; NLS, nuclear localization sequence; PEG, polyethylene glycol; SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis.