Glial Suppression of IMD/NF-κB Signaling in Healthy Flies Extends Active Lifespan
(A–C) The maximum lifespan of a hypomorphic allele of Dredd (DreddEP1214) showed a modest extension compared with w1118 controls. Nevertheless, midbrain sections (B) and quantification (C) of yw; dreddB118, w1118; relE20-null mutants and a w1118; dreddEP1412 hypomorphic mutant showed no difference in neurodegeneration compared with controls.
(D–G) RNAi-dependent silencing of rel in glia resulted in a very substantial extension of lifespan (D) and amelioration of the age-dependent decline in locomotor activity (E). Similarly, glial knockdown of Dredd and Imd resulted in lifespan extension (F) and amelioration of the age-dependent decline in locomotor activity (G).
Values shown are mean ± SEM. Asterisks denote statistically significant differences (∗p ≤ 0.05, ∗∗p ≤ 0.01, ∗∗∗∗p ≤ 0.0001).