Figure 6.

Glial Suppression of IMD/NF-κB Signaling in Healthy Flies Extends Active Lifespan

(A–C) The maximum lifespan of a hypomorphic allele of Dredd (Dredd^EP1214) showed a modest extension compared with w¹¹¹⁸ controls. Nevertheless, midbrain sections (B) and quantification (C) of yw; dredd^B118, w¹¹¹⁸; rel^E20-null mutants and a w¹¹¹⁸; dredd^EP1412 hypomorphic mutant showed no difference in neurodegeneration compared with controls.

(D–G) RNAi-dependent silencing of rel in glia resulted in a very substantial extension of lifespan (D) and amelioration of the age-dependent decline in locomotor activity (E). Similarly, glial knockdown of Dredd and Imd resulted in lifespan extension (F) and amelioration of the age-dependent decline in locomotor activity (G).

Values shown are mean ± SEM. Asterisks denote statistically significant differences (^∗p ≤ 0.05, ^∗∗p ≤ 0.01, ^∗∗∗∗p ≤ 0.0001).