Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Mar 2;104(3):412–427. doi: 10.1111/mmi.13637

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017 The Authors Molecular Microbiology Published by John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Processing of TbPSD.

A. Predicted processing of wild‐type (1) and mutated (2) TbPSD into α‐ and β‐subunits. MT, mitochondrial targeting signal; TMD, transmembrane domain; CS, cleavage site; WGSS/WGAS, amino acid motifs at predicted cleavage site; 3xHA, C‐terminal attachment of 3xHA tag.

B. SDS‐PAGE/immunoblot showing two bands representing the proenzyme (43.2 kDa) and the cleaved α‐subunit (7.4 kDa) (left lane), and a single band representing the uncleaved proenzyme after mutation of the recognition site (right lane).