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. 2017 Mar 2;104(3):412–427. doi: 10.1111/mmi.13637

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© 2017 The Authors Molecular Microbiology Published by John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Localization of TbPSD by immunofluorescence microscopy. Hemagglutinin (HA)‐tagged wild‐type (top and middle rows) and mutated forms (bottom row) of TbPSD were expressed in T. brucei procyclic (PCF; top and bottom rows) and bloodstream forms (BSF; middle row). Subcellular localization of TbPSD was analyzed by immunofluorescence microscopy using anti‐HA antibody and the mitochondrial dye MitoTracker. DNA (in blue) was stained with 4′,6′‐diamidino‐2‐phenylindole. Scale bars indicate 10 µm.