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. 2017 Mar 2;104(3):412–427. doi: 10.1111/mmi.13637

Figure 6.

Figure 6

Effect of TbPSD down‐regulation on mitochondria.

A, B. Mitochondrial structure and membrane potential were analyzed by immunofluorescence in procyclic (panel A) and bloodstream form (panel B) parasites. Mitochondria from control and TbPSD‐depleted trypanosomes (induced with tetracycline for 6 days) were live‐stained with MitoTracker, then cells were fixed on slides and co‐stained with an antibody against a mitochondrial marker protein (mtHSP70). DNA was stained with 4′,6′‐diamidino‐2‐phenylindole (in blue). Scale bars indicate 10 µm.

C, D. ATP production by oxidative phosphorylation (C) or at substrate level (D) was measured in digitonin extracts of control and TbPSD‐depleted trypanosomes (induced with tetracycline for 6 days). Antimycin and atractyloside were added to inhibit complex III (panel C) and ADP/ATP translocase (panel D), respectively. Data are from two independent experiments performed in duplicates. Error bars indicate means ± standard deviations. The asterisk indicates significant difference with control (*P < 0.0001, unpaired student's t test).