Figure 8.
Recombinant PS decarboxylase activity in E. coli.
A. Membranes from E. coli containing either no vector, the empty vector or the vector containing TbPSD or S. cerevisiae PSD1 (ScPSD1) for complementation were incubated with [3H]PS for 10 min. After extraction, lipids were analysed by high performance thin layer chromatography and radiolabeled PS and PE were detected by fluorography. Samples from two parallel experiments were applied.
B. Soluble (truncated) TbPSD was expressed and purified from E. coli and assayed with [3H]PS for 60 min in the same manner as the full length enzyme. Lipids were extracted and analysed as in panel A. The migration of PE and PS standards applied on the same plate is indicated in the left margins.