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Binominal distribution can be applied for calculation of the relative amount of the expected species (x = relative content of the trisulfide linked LC‐HC peptides)
| Species | Relative content |
|---|---|
| No trisulfide at both LC‐HC disulfide linkages | (1 − x)2 × 100% |
| Trisulfide at one LC‐HC disulfide linkage | x(1 − x) × 100% |
| Trisulfide at both LC‐HC disulfide linkages | x 2 × 100% |
