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. Author manuscript; available in PMC: 2017 Oct 3.
Published in final edited form as: Nat Neurosci. 2017 Apr 3;20(5):735–742. doi: 10.1038/nn.4538

Figure 1. Immunohistochemical verification of Cre-dependent ChR2 and eYFP expression in tyrosine hydroxylase-expression (TH+) neurons and fiber placements in the VTA.

Figure 1

Left images: 90% of YFP-expressing neurons (green) also expressed TH (red). Bottom images are an expansion of the region boxed at top. Right panel: Unilateral representation of the bilateral fiber placements and virus expression in each group. Fiber implants (black circles) were localized in the vicinity of eYFP (green) and ChR2 (blue) expression in VTA. Light shading represents the maximal and dark shading indicates the minimal spread of expression at each level. Scale bar = 20μm.