Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Apr 26;7(4):160327. doi: 10.1098/rsob.160327

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

PMC Copyright notice

Figure 8. — OiMacroD de-MARylation activity. (a) Extracted ion chromatograms of the monoisotopic mass of the ADP-ribose [M + H]⁺ adduct (560 m/z) for the ADP de-ribosylation reactions of OiMacroD WT and its mutants. (b) MS analysis of ADPr standard (i) and hPARP1 E988Q-OiMacroD WT reaction (ii). The 560.0 m/z corresponds to ADPr. (c) Ion abundance of mass 560.0 for OiMacroD WT and mutants in de-MARylation reactions. Error bars represent the standard deviation of three replicate runs. hPARP1 E988Q represents the mono-ADP-ribosylated protein, whereas untreated hPARP1 E988Q means the unmodified protein. The blank is the reaction buffer used to produce MARylated hPARP1 E988Q. OiMacroD WT and its mutants are designed as WT, N30A, G37V and D40A, respectively.