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. Author manuscript; available in PMC: 2017 May 3.
Published in final edited form as: Exp Biol Med (Maywood). 2014 Jul 3;239(9):1080–1095. doi: 10.1177/1535370214539232

Figure 3.

Figure 3

Limb bud mesenchymal micromass. Embryonic chick limb bud micromass cultured for 21 days and stained with Safranin O/Fast green (Saf O/FG), and immunostained for collagen type I (Col 1), collagen type II (Col 2), alkaline phosphatase (Alk Phos), and Indian hedgehog (IHH). Bar =50 μm, Inset Bar 100 μm. (A color version of this figure is available in the online journal.)