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MSCs chondrogenic differentiation in 3D. Human bone marrow derived MSCs were seeded within 1 mg/mL collagen type I gels and cultured for 21 days with TGF-β1 or TGF-β3 containing chondrogenic medium or control medium. Samples were processed for histology and stained with Safranin O/Fast green staining. Red staining indicates matrix sulfated glycosaminoglycan deposition. Bar =50 μm. (A color version of this figure is available in the online journal.)
